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Issue 11, 2012
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A systems biology approach to identify the signalling network regulated by Rho-GDI-γ during neural stem cell differentiation

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Abstract

Understanding the molecular mechanism that underlies the differentiation of neural stem cells (NSCs) is vital to develop regenerative medicines for neurological disorders. In our previous work, Rho-GDI-γ was found to be able to prompt neuronal differentiation when it was down regulated. However, it is unclear how Rho-GDI-γ regulates this differentiation process. Therefore, a novel systems biology approach is presented here to identify putative signalling pathways regulated by Rho-GDI-γ during NSC differentiation, and these pathways can provide insights into the NSC differentiation mechanisms. In particular, our proposed approach combines the predictive power of computational biology and molecular experiments. With different biological experiments, the genes in the computationally identified signalling network were validated to be indeed regulated by Rho-GDI-γ during the differentiation of NSCs. In particular, one randomly selected pathway involving Vcp, Mapk8, Ywhae and Ywhah was experimentally verified to be regulated by Rho-GDI-γ. These promising results demonstrate the effectiveness of our proposed systems biology approach, indicating the potential predictive power of integrating computational and experimental approaches.

Graphical abstract: A systems biology approach to identify the signalling network regulated by Rho-GDI-γ during neural stem cell differentiation

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Publication details

The article was received on 16 Apr 2012, accepted on 26 Jul 2012 and first published on 30 Jul 2012


Article type: Paper
DOI: 10.1039/C2MB25147G
Citation: Mol. BioSyst., 2012,8, 2916-2923

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    A systems biology approach to identify the signalling network regulated by Rho-GDI-γ during neural stem cell differentiation

    J. Wang, F. Hu, H. Cheng, X. Zhao and T. Wen, Mol. BioSyst., 2012, 8, 2916
    DOI: 10.1039/C2MB25147G

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