A PNA microarray for tomato genotyping

Tullia Tedeschi; Alessandro Calabretta; Mariangela Bencivenni; Alex Manicardi; Giandomenico Corrado; Martina Caramante; Roberto Corradini; Rosa Rao; Stefano Sforza; Rosangela Marchelli

doi:10.1039/C1MB05048F

A PNA microarray for tomato genotyping

Tullia Tedeschi,^a Alessandro Calabretta,^a Mariangela Bencivenni,^a Alex Manicardi,^a Giandomenico Corrado,^b Martina Caramante,^b Roberto Corradini,^a Rosa Rao,^b Stefano Sforza*^a and Rosangela Marchelli*^a

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* Corresponding authors

^a Dipartimento di Chimica Organica e Industriale, University of Parma, Parco Area delle Scienze 17a, Parma, Italy
E-mail: stefano.sforza@unipr.it
Fax: +39-0521-905472

^b Dipartimento di Scienze del Suolo, della Pianta, dell'Ambiente e delle Produzioni Animali, University of Napoli, Reggia di Portici-Via Università 100, Portici-Napoli, Italy

Abstract

The design and development of a PNA microarray designed for the simultaneous identification of several SNPs characteristic of seven different tomato varieties is described. Highly selective arginine-based monomer containing PNAs (Arg-PNAs) have been used in order to obtain very selective probes. Seven modified PNA probes were synthesised and their binding properties in solution were studied. PNA-microarrays based on these probes were prepared and applied to SNP discrimination in model experiments using oligonucleotide mixtures simulating the different sequences of the seven tomato varieties. The strength and the limitations of such a system for SNP recognition are thoroughly discussed.

Molecular BioSystems

A PNA microarray for tomato genotyping

Abstract

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A PNA microarray for tomato genotyping

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