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Issue 3, 2011
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iTRAQ-based profiling of grape berry exocarp proteins during ripening using a parallel mass spectrometric method

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Abstract

The 4-plex iTRAQ platform was utilized to analyze the protein profiles in four stages of grapevine berry skin ripening, from pre-veraison to fully ripening. Mass spectrometric data were acquired from three replicated analyses using a parallel acquisition method in an Orbitrap instrument by combining collision-induced dissociation (CID) and higher energy collision-induced dissociation (HCD) peptide ion fragmentations. As a result, the number of spectra suitable for peptide identification (either from CID or HCD) increased 5-fold in relation to those suitable for quantification (from HCD). Spectra were searched against an NCBInr protein database subset containing all the Vitis sequences, including those derived from whole genome sequencing. In general, 695 unique proteins were identified with more than one single peptide, and 513 of them were quantified. The sequence annotation and GO term enrichment analysis assisted by the automatic annotation tool Blast2GO permitted a pathway analysis which resulted in finding that biological processes and metabolic pathways de-regulated throughout ripening. A detailed analysis of the function-related proteins profiles helped discover a set of proteins of known Vitis gene origin as the potential candidates to play key roles in grapevine berry quality, growth regulation and disease resistance.

Graphical abstract: iTRAQ-based profiling of grape berry exocarp proteins during ripening using a parallel mass spectrometric method

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Supplementary files

Article information


Submitted
09 Sep 2010
Accepted
21 Oct 2010
First published
26 Nov 2010

Mol. BioSyst., 2011,7, 749-765
Article type
Paper

iTRAQ-based profiling of grape berry exocarp proteins during ripening using a parallel mass spectrometric method

M. J. Martínez-Esteso, J. Casado-Vela, S. Sellés-Marchart, F. Elortza, M. A. Pedreño and R. Bru-Martínez, Mol. BioSyst., 2011, 7, 749
DOI: 10.1039/C0MB00194E

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