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Issue 12, 2010
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Galactosyl conjugated N-succinyl-chitosan-graft-polyethylenimine for targeting gene transfer

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Abstract

Through incorporating lactobionic acid (LA) bearing a galactose group to N-succinyl-chitosan-graft-polyethylenimine (NSC-g-PEI), NSC-g-PEI-LA copolymers were synthesized as gene vectors with hepatocyte targeting properties. The molecular weight and composition of NSC-g-PEI-LA copolymers were characterized using gel permeation chromatography (GPC) and 1H nuclear magnetic resonance spectroscopy (1H NMR) respectively. Agarose gel electrophoresis assays showed good DNA binding ability of NSC-g-PEI-LA, and the particle size of the NSC-g-PEI-LA/DNA complexes were between 150 and 400 nm as determined by a Zeta sizer. The NSC-g-PEI-LA/DNA complexes observed by scanning electron microscopy (SEM) exhibited a compact and spherical morphology. The zeta potentials of these complexes were increased with the weight ratio of NSC-g-PEI-LA/DNA. NSC-g-PEI-LA has a lower cytotoxicity than PEI (25 kDa) and the toxicity decreased with increasing substitution of LA. The transfection efficiency of different complexes was evaluated by luciferase assay. Compared with PEI (25 kDa) and NSC-g-PEI/DNA, NSC-g-PEI-LA showed good transfection activity and cell specificity to HepG2 cells. The results suggested that NSC-g-PEI-LA has the potential to be used as a safe and effective targeting gene vector.

Graphical abstract: Galactosyl conjugated N-succinyl-chitosan-graft-polyethylenimine for targeting gene transfer

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Publication details

The article was received on 11 Jul 2010, accepted on 17 Aug 2010 and first published on 18 Oct 2010


Article type: Paper
DOI: 10.1039/C0MB00096E
Mol. BioSyst., 2010,6, 2529-2538

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    Galactosyl conjugated N-succinyl-chitosan-graft-polyethylenimine for targeting gene transfer

    B. Lu, D. Wu, H. Zheng, C. Quan, X. Zhang and R. Zhuo, Mol. BioSyst., 2010, 6, 2529
    DOI: 10.1039/C0MB00096E

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