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Issue 12, 2009
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Prediction of protein–protein interactions between Helicobacter pylori and a human host

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Abstract

A lack of information on proteinprotein interactions at the host–pathogen interface is impeding the understanding of the pathogenesis process. A recently developed, homology search-based method to predict proteinprotein interactions is applied to the gastric pathogen, Helicobacter pylori to predict the interactions between proteins of H. pylori and human proteinsin vitro. Many of the predicted interactions could potentially occur between the pathogen and its human host during pathogenesis as we focused mainly on the H. pyloriproteins that have a transmembrane region or are encoded in the pathogenic island and those which are known to be secreted into the human host. By applying the homology search approach to proteinprotein interaction databases DIP and iPfam, we could predict in vitro interactions for a total of 623 H. pyloriproteins with 6559 human proteins. The predicted interactions include 549 hypothetical proteins of as yet unknown function encoded in the H. pylorigenome and 13 experimentally verified secreted proteins. We have recognized 833 interactions involving the extracellular domains of transmembraneproteins of H. pylori. Structural analysis of some of the examples reveals that the interaction predicted by us is consistent with the structural compatibility of binding partners. Examples of interactions with discernible biological relevance are discussed.

Graphical abstract: Prediction of protein–protein interactions between Helicobacter pylori and a human host

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Supplementary files

Article information


Submitted
01 Apr 2009
Accepted
13 Jul 2009
First published
28 Sep 2009

Mol. BioSyst., 2009,5, 1630-1635
Article type
Paper

Prediction of proteinprotein interactions between Helicobacter pylori and a human host

N. Tyagi, O. Krishnadev and N. Srinivasan, Mol. BioSyst., 2009, 5, 1630
DOI: 10.1039/B906543C

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