High-content siRNA screening
Abstract
Very recent developments in instrumentation and image analysis have made microscopy applicable to high-throughput screening (HTS). For ‘High-Content Screening’ modern automated microscopy systems provide a throughput of up to 100 000 (confocal) images, with amazingly high resolution, of cells fluorescently stained using multiple colours that are imaged simultaneously during the screen. Image analysis tools provide multi-parametric pattern extraction and quantification on-the-fly. Big pharmaceutical companies have presented image-based screens of more than 100 000 compounds, while academia has published data on large
Numerous whole-genome