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Issue 8, 2021
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Isolation and recovery of extracellular vesicles using optically-induced dielectrophoresis on an integrated microfluidic platform

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Abstract

Cell-released, membrane-encapsulated extracellular vesicles (EVs) serve as a means of intercellular communication by delivering bioactive cargos including proteins, nucleic acids and lipids. EVs have been widely used for a variety of biomedical applications such as biomarkers for disease diagnosis and drug delivery vehicles for therapy. Herein, this study reports a novel method for label-free, contact-free isolation and recovery of EVs via optically-induced dielectrophoresis (ODEP) on a pneumatically-driven microfluidic platform with minimal human intervention. At an optimal driving frequency of 20 kHz and a voltage of 20 Vpp, an ODEP force from a 75 μm moving light beam was characterized to be 23.5–97.7 fN in 0.2 M sucrose solution. Furthermore, rapid enrichment of EVs with a small volume of only 27 pL in 32 s achieved an increase of 272-fold by dynamically shrinking circular light patterns. Moreover, EVs could be automatically isolated and recovered within 25 min, while achieving a releasing efficiency of 99.8% and a recovery rate of 52.2% by using an integrated microfluidics-based optically-induced EV isolation (OIEV) platform. Given the capacity of label-free, contact-free EV isolation, and automatic, easy-releasing EV recovery, this integrated OIEV platform provides a unique approach for EV-based disease diagnosis and drug delivery applications.

Graphical abstract: Isolation and recovery of extracellular vesicles using optically-induced dielectrophoresis on an integrated microfluidic platform

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Supplementary files

Article information


Submitted
05 Feb 2021
Accepted
12 Mar 2021
First published
15 Mar 2021

Lab Chip, 2021,21, 1475-1483
Article type
Paper

Isolation and recovery of extracellular vesicles using optically-induced dielectrophoresis on an integrated microfluidic platform

Y. Chen, C. P. Lai, C. Chen and G. Lee, Lab Chip, 2021, 21, 1475 DOI: 10.1039/D1LC00093D

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