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Issue 7, 2021

Paper-thin multilayer microfluidic devices with integrated valves

Author affiliations

Abstract

Integrated valve microfluidics has an unparalleled capability to automate rapid delivery of fluids at the nanoliter scale for high-throughput biological experimentation. However, multilayer soft lithography, which is used to fabricate valve-microfluidics, produces devices with a minimum thickness of around five millimeters. This form-factor limitation prevents the use of such devices in experiments with limited sample thickness tolerance such as 4-pi microscopy, stimulated Raman scattering microscopy, and many forms of optical or magnetic tweezer applications. We present a new generation of integrated valve microfluidic devices that are less than 300 μm thick, including the cover-glass substrate, that resolves the thickness limitation. This “thin-chip” was fabricated through a novel soft-lithography technique that produces on-chip micro-valves with the same functionality and reliability of traditional thick valve-microfluidic devices despite the orders of magnitude reduction in thickness. We demonstrated the advantage of using our thin-chip over traditional thick devices to automate fluid control while imaging on a high-resolution inverted microscope. First, we demonstrate that the thin-chip provides an improved signal to noise when imaging single cells with two-color stimulated Raman scattering (SRS). We then demonstrated how the thin-chip can be used to simultaneously perform on-chip magnetic manipulation of beads and fluorescent imaging. This study reveals the potential of our thin-chip in high-resolution imaging, sorting, and bead capture-based single-cell multi-omics applications.

Graphical abstract: Paper-thin multilayer microfluidic devices with integrated valves

Supplementary files

Article information


Submitted
02 Dec 2020
Accepted
03 Mar 2021
First published
03 Mar 2021

This article is Open Access

Lab Chip, 2021,21, 1287-1298
Article type
Communication

Paper-thin multilayer microfluidic devices with integrated valves

S. Kim, G. Dorlhiac, R. Cotrim Chaves, M. Zalavadia and A. Streets, Lab Chip, 2021, 21, 1287 DOI: 10.1039/D0LC01217C

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