Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 21st October 2020 from 07:00 AM to 07:00 PM (BST).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 18, 2020
Previous Article Next Article

SNAPflex: a paper-and-plastic device for instrument-free RNA and DNA extraction from whole blood

Author affiliations

Abstract

Nucleic acid amplification tests (NAATs), which amplify and detect pathogen nucleic acids, are vital methods to diagnose diseases, particularly in cases where patients exhibit low levels of infection. For many blood-borne pathogens such as HIV or Plasmodium falciparum, it is necessary to first extract pathogen RNA or DNA from patient blood prior to NAAT analysis. Traditional nucleic acid extraction methods are expensive, resource-intensive and are often difficult to deploy to resource-limited areas where many blood-borne infections are widespread. Here, we describe a portable, paper-and-plastic device, called SNAPflex, for instrument-free nucleic acid extraction from whole blood, which builds upon our previous work for RNA extraction using a pressure-driven extraction system. SNAPflex shows improved HIV RNA extraction from simulated patient samples compared to traditional extraction methods as well as long-term stability of extracted RNA without the need for cold storage. We further demonstrated successful extraction and recovery of P. falciparum DNA from cultured parasites in whole blood. SNAPflex was designed to be easily manufacturable and deployable to resource-limited settings.

Graphical abstract: SNAPflex: a paper-and-plastic device for instrument-free RNA and DNA extraction from whole blood

Back to tab navigation

Supplementary files

Article information


Submitted
17 Mar 2020
Accepted
31 Jul 2020
First published
04 Aug 2020

Lab Chip, 2020,20, 3386-3398
Article type
Paper

SNAPflex: a paper-and-plastic device for instrument-free RNA and DNA extraction from whole blood

N. Kolluri, N. Albarran, A. Fan, A. Olson, M. Sagar, A. Young, J. Gomez-Marquez and C. M. Klapperich, Lab Chip, 2020, 20, 3386
DOI: 10.1039/D0LC00277A

Social activity

Search articles by author

Spotlight

Advertisements