Issue 5, 2020

Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML

Abstract

We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. Our single-cell microwell array platform quantifies protein phosphorylation using enzymatic signal amplification and exploiting Michaelis–Menten kinetics. Our method provides a two-fold increase in resolution compared to conventional flow cytometry.

Graphical abstract: Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML

Supplementary files

Article information

Article type
Paper
Submitted
09 Dec 2019
Accepted
29 Jan 2020
First published
29 Jan 2020

Lab Chip, 2020,20, 942-948

Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML

J. Yu, K. Oh, S. Moorthi, L. Li, H. H. Strey, M. Schuster, C. Luberto, P. Quan and E. Brouzes, Lab Chip, 2020, 20, 942 DOI: 10.1039/C9LC01213C

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