Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 5, 2020
Previous Article Next Article

Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML

Author affiliations

Abstract

We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. Our single-cell microwell array platform quantifies protein phosphorylation using enzymatic signal amplification and exploiting Michaelis–Menten kinetics. Our method provides a two-fold increase in resolution compared to conventional flow cytometry.

Graphical abstract: Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML

Back to tab navigation

Supplementary files

Article information


Submitted
09 Dec 2019
Accepted
29 Jan 2020
First published
29 Jan 2020

Lab Chip, 2020,20, 942-948
Article type
Paper

Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML

J. Yu, K. Oh, S. Moorthi, L. Li, H. H. Strey, M. Schuster, C. Luberto, P. Quan and E. Brouzes, Lab Chip, 2020, 20, 942
DOI: 10.1039/C9LC01213C

Social activity

Search articles by author

Spotlight

Advertisements