Jump to main content
Jump to site search

Issue 21, 2019
Previous Article Next Article

Synchrotron infrared nanospectroscopy on a graphene chip

Author affiliations

Abstract

A recurring goal in biology and biomedicine research is to access the biochemistry of biological processes in liquids that represent the environmental conditions of living organisms. These demands are becoming even more specific as microscopy techniques are fast evolving in the era of single cell analysis. In the modality of chemical probes, synchrotron infrared spectroscopy (μ-FTIR) is a technique that is extremely sensitive to vibrational responses of materials; however, the classical optical limits prevent the technique to access the biochemistry of specimens at the subcellular level. In addition, due to the intricate environmental requirements and strong infrared absorption of water, μ-FTIR of bioprocesses in liquids remains highly challenging. In phase with these challenges, on-chip liquid cells emerge as a versatile alternative to control the water thickness while providing a biocompatible chemical environment for analytical analyses. In this work we report the development of a liquid platform specially designed for nanoscale infrared analysis of biomaterials in wet environments. A key advantage of our designed platform is the use of graphene as an optical window that interfaces wet and dry environments in the liquid cell. By combining near-field optical microscopy and synchrotron infrared radiation, we measure the nanoscale fingerprint IR absorbance of a variety of liquids often used in biological studies. Further, we demonstrate the feasibility of the platform for the chemical analysis of protein clusters immersed in water with a clear view of the proteins' secondary structure signatures. The simplicity of the proposed platform combined with the high quality of our data makes our findings a template for future microfluidic devices targeting dynamic nanoscale-resolved chemical analysis.

Graphical abstract: Synchrotron infrared nanospectroscopy on a graphene chip

Back to tab navigation

Publication details

The article was received on 16 Jul 2019, accepted on 07 Sep 2019 and first published on 13 Sep 2019


Article type: Paper
DOI: 10.1039/C9LC00686A
Lab Chip, 2019,19, 3678-3684

  •   Request permissions

    Synchrotron infrared nanospectroscopy on a graphene chip

    L. M. Meireles, I. D. Barcelos, G. A. Ferrari, P. A. A. de A. Neves, R. O. Freitas and R. G. Lacerda, Lab Chip, 2019, 19, 3678
    DOI: 10.1039/C9LC00686A

Search articles by author

Spotlight

Advertisements