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Issue 24, 2019
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Cell lysis via acoustically oscillating sharp edges

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In this article, we demonstrate an acoustofluidic device for cell lysis using the acoustic streaming effects induced by acoustically oscillating sharp-edged structures. The acoustic streaming locally generates high shear forces that can mechanically rupture cell membranes. With the acoustic-streaming-derived shear forces, our acoustofluidic device can perform cell lysis in a continuous, reagent-free manner, with a lysis efficiency of more than 90% over a range of sample flow rates. We demonstrate that our acoustofluidic lysis device works well on both adherent and non-adherent cells. We also validate it using clinically relevant samples such as red blood cells infected with malarial parasites. Additionally, the unique capability of our acoustofluidic device was demonstrated by performing downstream protein analysis and gene profiling without additional washing steps post-lysis. Our device is simple to fabricate and operate while consuming a relatively low volume of samples. These advantages and other features including the reagent-free nature and controllable lysis efficiency make our platform valuable for many biological and biomedical applications, particularly for the development of point-of-care platforms.

Graphical abstract: Cell lysis via acoustically oscillating sharp edges

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Article information

26 May 2019
05 Nov 2019
First published
13 Nov 2019

Lab Chip, 2019,19, 4021-4032
Article type
Author version available

Cell lysis via acoustically oscillating sharp edges

Z. Wang, P. Huang, C. Chen, H. Bachman, S. Zhao, S. Yang and T. J. Huang, Lab Chip, 2019, 19, 4021
DOI: 10.1039/C9LC00498J

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