Jump to main content
Jump to site search

Issue 9, 2019
Previous Article Next Article

Traction microscopy with integrated microfluidics: responses of the multi-cellular island to gradients of HGF

Author affiliations

Abstract

Collective cellular migration plays a central role in development, regeneration, and metastasis. In these processes, mechanical interactions between cells are fundamental but measurement of these interactions is often hampered by technical limitations. To overcome some of these limitations, here we describe a system that integrates microfluidics with traction microscopy (TM). Using this system we can measure simultaneously, and in real time, migration speeds, tractions, and intercellular tension throughout an island of confluent Madin–Darby canine kidney (MDCK) cells. The cell island is exposed to hepatocyte growth factor (HGF) at a controlled gradient of concentrations; HGF is known to elicit epithelial-to-mesenchymal transition (EMT) and cell scattering. As expected, the rate of expansion of the cell island was dependent on the concentration of HGF. Higher concentrations of HGF reduced intercellular tensions, as expected during EMT. A novel finding, however, is that the effects of HGF concentration and its gradient were seen within an island. This integrated experimental system thus provides an integrated tool to better understand cellular forces during collective cellular migration under chemical gradients.

Graphical abstract: Traction microscopy with integrated microfluidics: responses of the multi-cellular island to gradients of HGF

Back to tab navigation

Supplementary files

Article information


Submitted
19 Feb 2019
Accepted
26 Mar 2019
First published
27 Mar 2019

Lab Chip, 2019,19, 1579-1588
Article type
Paper

Traction microscopy with integrated microfluidics: responses of the multi-cellular island to gradients of HGF

H. Jang, J. Kim, J. H. Shin, J. J. Fredberg, C. Y. Park and Y. Park, Lab Chip, 2019, 19, 1579
DOI: 10.1039/C9LC00173E

Social activity

Search articles by author

Spotlight

Advertisements