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Issue 8, 2019
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Hydrodynamic shuttling for deterministic high-efficiency multiple single-cell capture in a microfluidic chip

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Abstract

Studies on cellular heterogeneity have emerged as a powerful approach for developing new strategies to treat diseases including cancer. However, it is difficult to set up an in vitro co-culture experiment to study the interaction of individual live cells. In this paper, we report a hydrodynamic shuttling chip (HSC) which can deterministically capture single cells into microfluidic chambers to set up multiple single-cell co-culture experiments in which individual live cells can be microscopically observed. Using this chip device, we demonstrated a triple single-cell culture of oral squamous cell carcinoma and lymphatic endothelial cells to observe the effect of cell–cell interaction on the cell motility. Triple, single-cell pairing efficiency by our HSC device was eightfold higher than that of the probabilistic method. Using this HSC device, we were able to perform triple-culture experiments to show the cell type-dependent motility of oral squamous cell carcinoma and lymphatic endothelial cells, which was not observed in co-culture experiments.

Graphical abstract: Hydrodynamic shuttling for deterministic high-efficiency multiple single-cell capture in a microfluidic chip

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Supplementary files

Article information


Submitted
10 Jan 2019
Accepted
11 Mar 2019
First published
15 Mar 2019

Lab Chip, 2019,19, 1370-1377
Article type
Paper

Hydrodynamic shuttling for deterministic high-efficiency multiple single-cell capture in a microfluidic chip

C. He, Y. Chen, S. Wang and C. Hsu, Lab Chip, 2019, 19, 1370
DOI: 10.1039/C9LC00036D

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