Issue 8, 2019

Multiparameter antibiotic resistance detection based on hydrodynamic trapping of individual E. coli

Abstract

There is an urgent need to develop novel methods for assessing the response of bacteria to antibiotics in a timely manner. Antibiotics are traditionally assessed via their effect on bacteria in a culture medium, which takes 24–48 h and exploits only a single parameter, i.e. growth. Here, we present a multiparameter approach at the single-cell level that takes approximately an hour from spiking the culture to correctly classify susceptible and resistant strains. By hydrodynamically trapping hundreds of bacteria, we simultaneously monitor the evolution of motility and morphology of individual bacteria upon drug administration. We show how this combined detection method provides insights into the activity of antimicrobials at the onset of their action which single parameter and traditional tests cannot offer. Our observations complement the current growth-based methods and highlight the need for future antimicrobial susceptibility tests to take multiple parameters into account.

Graphical abstract: Multiparameter antibiotic resistance detection based on hydrodynamic trapping of individual E. coli

Supplementary files

Article information

Article type
Paper
Submitted
21 Dec 2018
Accepted
15 Feb 2019
First published
14 Mar 2019
This article is Open Access
Creative Commons BY license

Lab Chip, 2019,19, 1417-1426

Multiparameter antibiotic resistance detection based on hydrodynamic trapping of individual E. coli

G. Pitruzzello, S. Thorpe, S. Johnson, A. Evans, H. Gadêlha and T. F. Krauss, Lab Chip, 2019, 19, 1417 DOI: 10.1039/C8LC01397G

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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