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Issue 4, 2019
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A microfluidic sensor for detecting chlorophenols using cross-linked enzyme aggregates (CLEAs)

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Abstract

Chlorophenols have a strong medicinal smell and can be detected by the human nose at parts-per-million levels. Therefore, continuous monitoring of chlorophenols in water supplies is highly important. Herein, we reported a microfluidic sensor which can be used to detect 2,4-dichlorophenol (2,4-DCP) in real time with a limit of detection of around 0.1 ppm. The microfluidic sensor is a membrane-less galvanic cell which consists of two laminar flows running in parallel inside a straight channel. The sensor measures the potential difference between a solution containing 2,4-DCP and a reference solution containing acetate buffer. In a continuous-flow mode, the cell potential is proportional to the concentration of 2,4-DCP. To render specificity for the sensor, we incorporate a pre-treatment section where the incoming solution containing 2,4-DCP is split into two streams. One of the streams is brought into contact with cross-linked laccase aggregates (which catalyzes the hydrolysis of 2,4-DCP) and the second stream is taken as a reference solution. By comparing the potential difference between the two streams, we can determine the concentration of 2,4-DCP with high specificity. The microfluidic sensor platform is potentially useful for real-time detection of micropollutants present in aquatic systems with high sensitivity and specificity.

Graphical abstract: A microfluidic sensor for detecting chlorophenols using cross-linked enzyme aggregates (CLEAs)

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Publication details

The article was received on 07 Oct 2018, accepted on 28 Dec 2018 and first published on 02 Jan 2019


Article type: Paper
DOI: 10.1039/C8LC01065J
Lab Chip, 2019,19, 634-640

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    A microfluidic sensor for detecting chlorophenols using cross-linked enzyme aggregates (CLEAs)

    W. F. Ho, L. T. Nguyen and K. Yang, Lab Chip, 2019, 19, 634
    DOI: 10.1039/C8LC01065J

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