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Issue 22, 2018
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SD-chip enabled quantitative detection of HIV RNA using digital nucleic acid sequence-based amplification (dNASBA)

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Abstract

Quantitative detection of RNA is important in molecular biology and clinical diagnostics. Nucleic acid sequence-based amplification (NASBA), a single-step method to amplify single-stranded RNA, is attractive for use in point-of-care (POC) diagnostics because it is an isothermal technique that is as sensitive as RT-PCR with a shorter reaction time. However, NASBA is limited in its ability to provide accurate quantitative information, such as viral load or RNA copy number. Here we test a digital format of NASBA (dNASBA) using a self-digitization (SD) chip platform, and apply it to quantifying HIV-1 RNA. We demonstrate that dNASBA is more sensitive and accurate than the real-time quantitative NASBA, and can be used to quantify HIV-1 RNA in plasma samples. Digital NASBA is thus a promising POC diagnostics tool for use in resource-limited settings.

Graphical abstract: SD-chip enabled quantitative detection of HIV RNA using digital nucleic acid sequence-based amplification (dNASBA)

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Supplementary files

Article information


Submitted
10 Sep 2018
Accepted
12 Oct 2018
First published
17 Oct 2018

Lab Chip, 2018,18, 3501-3506
Article type
Paper

SD-chip enabled quantitative detection of HIV RNA using digital nucleic acid sequence-based amplification (dNASBA)

J. Wang, J. E. Kreutz, A. M. Thompson, Y. Qin, A. M. Sheen, J. Wang, L. Wu, S. Xu, M. Chang, D. N. Raugi, R. A. Smith, G. S. Gottlieb and D. T. Chiu, Lab Chip, 2018, 18, 3501
DOI: 10.1039/C8LC00956B

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