Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.

Issue 20, 2018
Previous Article Next Article

A single-cell translocation and secretion assay (TransSeA)

Author affiliations


Understanding biological heterogeneity at the single cell level is required for advancing insights into the complexity of human physiology and diseases. While advances in technological and analytical methods have afforded unprecedented glimpses of this heterogeneity, the information captured to date largely represents one-time “snap” shots of single cell physiology. To address the limits of existing methods and to accelerate discoveries from single cell studies, we developed a single-cell translocation and secretion assay (TransSeA) that supports time lapse analysis, enables molecular cargo analysis of secretions such as extracellular vesicles (EVs) from single cells, allows massively parallel single cell transfer according to user-defined cell selection criteria, and supports tracking of phenotypes between parental and progeny cells derived from single cells. To demonstrate the unique capabilities and efficiencies of the assay, we present unprecedented single cell studies related to cell secretions, EV cargos and cell intrinsic properties. Although used as examples to demonstrate the feasibility and versatility of the technology, the studies already provided insights into key unanswered questions such as the microRNAs carried by EVs, the relationships between EV secretion rates and gene expressions, and the spontaneous, trans-generational phenotypic changes in EV secretion between parental and progeny cells.

Graphical abstract: A single-cell translocation and secretion assay (TransSeA)

Back to tab navigation

Publication details

The article was received on 08 Aug 2018, accepted on 27 Aug 2018 and first published on 28 Aug 2018

Article type: Paper
DOI: 10.1039/C8LC00821C
Citation: Lab Chip, 2018,18, 3154-3162

  •   Request permissions

    A single-cell translocation and secretion assay (TransSeA)

    W. Cai, Y. Chiu, V. Ramakrishnan, Y. Tsai, C. Chen and Y. Lo, Lab Chip, 2018, 18, 3154
    DOI: 10.1039/C8LC00821C

Search articles by author