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Issue 13, 2018
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Retrieving acoustic energy densities and local pressure amplitudes in microfluidics by holographic time-lapse imaging

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Abstract

The development of techniques able to characterize and map the pressure field is crucial for the widespread use of acoustofluidic devices in biotechnology and lab-on-a-chip platforms. In fact, acoustofluidic devices are powerful tools for driving precise manipulation of microparticles and cells in microfluidics in non-contact modality. Here, we report a full and accurate characterization of the movement of particles subjected to acoustophoresis in a microfluidic environment by holographic imaging. The particle displacement along the direction of the ultrasound wave propagation, coinciding with the optical axis, is observed and investigated. Two resonance frequencies are explored, varying for each the amplitude of the applied signal. The trajectories of individual tracers, accomplished by holographic measurements, are fitted with the theoretical model thus allowing the retrieval of the acoustic energy densities and pressure amplitudes through full holographic analysis. The absence of prior calibration, being independent of the object shape and the possibility of implementing automatic analysis make the use of holography very appealing for applications in devices for biotechnologies.

Graphical abstract: Retrieving acoustic energy densities and local pressure amplitudes in microfluidics by holographic time-lapse imaging

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Supplementary files

Article information


Submitted
07 Feb 2018
Accepted
25 May 2018
First published
28 May 2018

Lab Chip, 2018,18, 1921-1927
Article type
Paper

Retrieving acoustic energy densities and local pressure amplitudes in microfluidics by holographic time-lapse imaging

T. Cacace, V. Bianco, M. Paturzo, P. Memmolo, M. Vassalli, M. Fraldi, G. Mensitieri and P. Ferraro, Lab Chip, 2018, 18, 1921
DOI: 10.1039/C8LC00149A

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