Jump to main content
Jump to site search

Issue 24, 2017

Performing multi-step chemical reactions in microliter-sized droplets by leveraging a simple passive transport mechanism

Author affiliations

Abstract

Despite the increasing importance of positron emission tomography (PET) imaging in research and clinical management of disease, access to myriad new radioactive tracers is severely limited due to their short half-lives (which requires daily production) and the high cost and complexity of tracer production. The application of droplet microfluidics based on electrowetting-on-dielectric (EWOD) to the field of radiochemistry can significantly reduce the amount of radiation shielding necessary for safety and the amount of precursor and other reagents needed for the synthesis. Furthermore, significant improvements in the molar activity of the tracers have been observed. However, widespread use of this technology is currently hindered in part by the high cost of prototype chips and the operating complexity. To address these issues, we developed a novel microfluidic device based on patterned wettability for multi-step radiochemical reactions in microliter droplets and implemented automated systems for reagent loading and collection of the crude product after synthesis. In this paper, we describe a simple and inexpensive method for fabricating the chips, demonstrate the feasibility of prototype chips for performing multi-step radiochemical reactions to produce the PET tracers [18F]fallypride and [18F]FDG, and further show that synthesized [18F]fallypride can be used for in vivo mouse imaging.

Graphical abstract: Performing multi-step chemical reactions in microliter-sized droplets by leveraging a simple passive transport mechanism

Supplementary files

Article information


Submitted
19 Sep 2017
Accepted
12 Nov 2017
First published
14 Nov 2017

Lab Chip, 2017,17, 4342-4355
Article type
Paper

Performing multi-step chemical reactions in microliter-sized droplets by leveraging a simple passive transport mechanism

J. Wang, P. H. Chao, S. Hanet and R. M. van Dam, Lab Chip, 2017, 17, 4342 DOI: 10.1039/C7LC01009E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements