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Issue 20, 2016
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Fully automated chemiluminescence detection using an electrified-Lab-on-a-Disc (eLoaD) platform

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Abstract

Typical Lab-on-a-Disc (LoaD) platforms cannot make a continuous measurement while the disc is spinning; this drawback means that the disc usually must be stopped and aligned with a sensor. This can result in measurement errors in time-dependent assays along with inaccuracies due to liquid displacement and bubble formation in the absence of a stabilising centrifugal field. This paper presents a novel concept for a wirelessly electrified-Lab-on-a-Disc (eLoaD) platform that allows continuous measurement of experimental parameters while the disc is spinning. This platform incorporates all the components needed for measurement within the rotating frame of reference, and bidirectional transmission of data outside this reference frame, thus allowing for online measurement independent of the rotation of the disc. The eLoaD platform is conceived in a modular manner whereby an interchangeable and non-disposable ‘Application Disc’ can be fitted to the eLoaD platform and so the system can be adapted for a range of optical, electrochemical and other measurement types. As an application example, optical readout, using the Application Disc fitted with a silicon photomultiplier, is demonstrated using a tagged chemiluminescent antibody, which is commonly used, for instance, in ELISA assays. The precision of the eLoaD platform is >94%, while its accuracy, when compared to a commercial benchtop luminometer, is higher than 96%. The modular design of this platform will permit extension of this technology to many other LoaD applications.

Graphical abstract: Fully automated chemiluminescence detection using an electrified-Lab-on-a-Disc (eLoaD) platform

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Article information


Submitted
29 Jul 2016
Accepted
06 Sep 2016
First published
06 Sep 2016

This article is Open Access

Lab Chip, 2016,16, 4002-4011
Article type
Paper

Fully automated chemiluminescence detection using an electrified-Lab-on-a-Disc (eLoaD) platform

S. M. T. Delgado, D. J. Kinahan, F. S. Sandoval, L. A. N. Julius, N. A. Kilcawley, J. Ducrée and D. Mager, Lab Chip, 2016, 16, 4002 DOI: 10.1039/C6LC00973E

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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