Issue 18, 2016

Highly efficient sample stacking by enhanced field amplification on a simple paper device

Abstract

We present a novel electrokinetic stacking (ES) method based on field amplification on a simple paper device for sample preconcentration. With voltage application, charged probe ions in a solution of lower conductivity stack and form a narrow band at the boundary between the sample and the background electrolyte of higher conductivity. The stacking band appears quickly and stabilizes in a few minutes. With this ES method, three orders of magnitude signal improvement was successfully achieved for both a fluorescein probe and a double-stranded DNA within 300 s. This enhanced stacking efficiency is attributed to a focusing effect due to the balance between electromigration and counter electroosmotic flow. We also applied this ES method to other low-cost fiber substrates such as cloth and thread. Such a simple and highly efficient ES method will find wide applications in the development of sensitive paper-based analytical devices (PADs), especially for low-cost point-of-care testing (POCT).

Graphical abstract: Highly efficient sample stacking by enhanced field amplification on a simple paper device

Supplementary files

Article information

Article type
Technical Innovation
Submitted
14 May 2016
Accepted
05 Aug 2016
First published
05 Aug 2016

Lab Chip, 2016,16, 3460-3465

Highly efficient sample stacking by enhanced field amplification on a simple paper device

B. Ma, Y. Song, J. Niu and Z. Wu, Lab Chip, 2016, 16, 3460 DOI: 10.1039/C6LC00633G

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