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Issue 9, 2016
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Mass spectrometry-based monitoring of millisecond protein–ligand binding dynamics using an automated microfluidic platform

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Abstract

Characterizing protein–ligand binding dynamics is crucial for understanding protein function and for developing new therapeutic agents. We present a novel microfluidic platform that features rapid mixing of protein and ligand solutions, variable incubation times, and an integrated electrospray ionization source for mass spectrometry-based monitoring of protein–ligand binding dynamics. This platform offers many advantages, including solution-based binding, label-free detection, automated operation, rapid mixing, and low sample consumption.

Graphical abstract: Mass spectrometry-based monitoring of millisecond protein–ligand binding dynamics using an automated microfluidic platform

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Article information


Submitted
09 Feb 2016
Accepted
17 Mar 2016
First published
24 Mar 2016

Lab Chip, 2016,16, 1544-1548
Article type
Communication
Author version available

Mass spectrometry-based monitoring of millisecond protein–ligand binding dynamics using an automated microfluidic platform

Y. Cong, S. Katipamula, C. D. Trader, D. J. Orton, T. Geng, E. S. Baker and R. T. Kelly, Lab Chip, 2016, 16, 1544
DOI: 10.1039/C6LC00183A

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