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Issue 9, 2016
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One-photon and two-photon stimulation of neurons in a microfluidic culture system

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Abstract

In this study, we demonstrate a novel platform for optical stimulation of neural circuits combined with a microfluidic culture method and microelectrode array measurements. Neuron-on-a-chip was designed and fabricated to isolate axons without a soma or dendrite. Thus, it is readily able to manipulate the neuronal alignment and to investigate the neuronal activity at the locations we want to observe. We adapted the optical stimulation technique to the arranged neurons to generate the neuronal signals in a non-invasive fashion. A blue light-emitting diode and a femtosecond laser with 780 nm center wavelength were used for neuronal activation and the corresponding neuronal signals were measured by MEAs at the same time. We found that one-photon light via caged glutamate provoked periodic spiking. In contrast, the femtosecond pulse irradiation generated repetitive firing at constant rates. Response times of one-photon and two-photon stimulation were around 200 ms and 50 ms, respectively. We also quantified neural responses, by varying optical parameters such as exposure time and irradiation power.

Graphical abstract: One-photon and two-photon stimulation of neurons in a microfluidic culture system

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Supplementary files

Article information


Submitted
16 Jan 2016
Accepted
17 Mar 2016
First published
23 Mar 2016

Lab Chip, 2016,16, 1684-1690
Article type
Paper

One-photon and two-photon stimulation of neurons in a microfluidic culture system

J. M. Jang, J. Lee, H. Kim, N. L. Jeon and W. Jung, Lab Chip, 2016, 16, 1684
DOI: 10.1039/C6LC00065G

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