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Issue 8, 2016
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A microfluidic array for real-time live-cell imaging of human and rodent pancreatic islets

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Abstract

In this study, we present a microfluidic array for high-resolution imaging of individual pancreatic islets. The device is based on hydrodynamic trapping principle and enables real-time analysis of islet cellular responses to insulin secretagogues. This device has significant advantages over our previously published perifusion chamber device including significantly increased analytical power and assay sensitivity, as well as improved spatiotemporal resolution. The islet array, with live-cell multiparametric imaging integration, provides a better tool to understand the physiological and pathophysiological changes of pancreatic islets through the analysis of single islet responses. This platform demonstrates the feasibility of array-based islet cellular analysis and opens up a new modality to conduct informative and quantitive evaluation of islets and cell-based screening for new diabetes treatments.

Graphical abstract: A microfluidic array for real-time live-cell imaging of human and rodent pancreatic islets

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Supplementary files

Article information


Submitted
29 Sep 2015
Accepted
04 Mar 2016
First published
04 Mar 2016

Lab Chip, 2016,16, 1466-1472
Article type
Paper
Author version available

A microfluidic array for real-time live-cell imaging of human and rodent pancreatic islets

M. Nourmohammadzadeh, Y. Xing, J. W. Lee, M. A. Bochenek, J. E. Mendoza-Elias, J. J. McGarrigle, E. Marchese, Y. Chun-Chieh, D. T. Eddington, J. Oberholzer and Y. Wang, Lab Chip, 2016, 16, 1466
DOI: 10.1039/C5LC01173F

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