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Issue 3, 2016
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A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis

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Abstract

Tumor-derived circulating exosomes, enriched with a group of tumor antigens, have been recognized as a promising biomarker source for cancer diagnosis via a less invasive procedure. Quantitatively pinpointing exosome tumor markers is appealing, yet challenging. In this study, we developed a simple microfluidic approach (ExoSearch) which provides enriched preparation of blood plasma exosomes for in situ, multiplexed detection using immunomagnetic beads. The ExoSearch chip offers a robust, continuous-flow design for quantitative isolation and release of blood plasma exosomes in a wide range of preparation volumes (10 μL to 10 mL). We employed the ExoSearch chip for blood-based diagnosis of ovarian cancer by multiplexed measurement of three exosomal tumor markers (CA-125, EpCAM, CD24) using a training set of ovarian cancer patient plasma, which showed significant diagnostic power (a.u.c. = 1.0, p = 0.001) and was comparable with the standard Bradford assay. This work provides an essentially needed platform for utilization of exosomes in clinical cancer diagnosis, as well as fundamental exosome research.

Graphical abstract: A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis

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Supplementary files

Article information


Submitted
16 Sep 2015
Accepted
20 Nov 2015
First published
20 Nov 2015

This article is Open Access

Lab Chip, 2016,16, 489-496
Article type
Paper

A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis

Z. Zhao, Y. Yang, Y. Zeng and M. He, Lab Chip, 2016, 16, 489 DOI: 10.1039/C5LC01117E

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