Jump to main content
Jump to site search

Issue 17, 2015
Previous Article Next Article

Research highlights: nanopore protein detection and analysis

Author affiliations

Abstract

In this article we highlight recent work using nanopores to detect and study proteins. Nanopores are excellent single molecule sensors, capable of rapidly characterizing small molecules with relatively modest instrumentation requirements. Although the vast majority of recent effort and attention surrounding nanopores has centered on detection and sequencing of nucleic acids, proteins represent a more difficult and diverse analyte population, with a wide range of sizes, structures, charges, among other characteristics. Nanopores can be used to detect the presence of proteins of interest as well as to study their enzymatic activity, binding to ligands, and secondary structure. We highlight new work describing detection of specific protein species in solution by coupling them to a strand of carrier DNA that is used to electrophoretically transport the proteins through conical glass nanopores. Additionally, we spotlight another approach for nanopore detection of protein and other analytes through detection of their binding to aptamers—measurements which were quantitative to pM concentrations. Finally, we highlight studies in which protein secondary structure and folding energetics were studied through the use of an unfoldase enzyme coupled to a protein nanopore, a technique capable of detecting the effects of single amino acid mutations on the stability of the folded protein.

Graphical abstract: Research highlights: nanopore protein detection and analysis

Back to tab navigation

Article information


First published
14 Jul 2015

Lab Chip, 2015,15, 3424-3427
Article type
Highlight

Research highlights: nanopore protein detection and analysis

S. Acharya, S. Edwards and J. Schmidt, Lab Chip, 2015, 15, 3424
DOI: 10.1039/C5LC90076J

Social activity

Search articles by author

Spotlight

Advertisements