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Issue 20, 2015
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McCLEC, a robust and stable enzymatic based microreactor platform

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Abstract

A microfluidic chip for cross-linked enzyme crystals (McCLEC) is presented and demonstrated to be a stable, reusable and robust biocatalyst-based device with very promising biotechnological applications. The cost-effective microfluidic platform allows in situ crystallization, cross-linking and enzymatic reaction assays on a single device. A large number of enzymatic reuses of the McCLEC platform were achieved and a comparative analysis is shown illustrating the efficiency of the process and its storage stability for more than one year.

Graphical abstract: McCLEC, a robust and stable enzymatic based microreactor platform

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Article information


Submitted
03 Jul 2015
Accepted
20 Aug 2015
First published
20 Aug 2015

This article is Open Access

Lab Chip, 2015,15, 4083-4089
Article type
Paper
Author version available

McCLEC, a robust and stable enzymatic based microreactor platform

M. Conejero-Muriel, I. Rodríguez-Ruiz, S. Martínez-Rodríguez, A. Llobera and J. A. Gavira, Lab Chip, 2015, 15, 4083
DOI: 10.1039/C5LC00776C

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