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Issue 10, 2015
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Direct detection and drug-resistance profiling of bacteremias using inertial microfluidics

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Abstract

Detection of bacteria in bloodstream infections and their antibiotic susceptibility patterns is critical to guide therapeutic decision-making for optimal patient care. Current culture-based assays are too slow (>48 h), leading to excessive up-front use of broad-spectrum antibiotics and/or incorrect antibiotic choices due to resistant bacteria, each with deleterious consequences for patient care and public health. To approach this problem, we describe a method to rapidly isolate bacteria from whole blood using inertial microfluidics and directly determine pathogen identity and antibiotic susceptibility with hybridization-based RNA detection. Using the principle of Dean flow fractionation, bacteria are separated from host blood cells in a label-free separation method with efficient recovery of even low abundance bacteria. Ribosomal RNA detection can then be applied for direct identification of low abundance pathogens (~100 per mL) from blood without culturing or enzymatic amplification. Messenger RNA detection of antibiotic-responsive transcripts after brief drug exposure permits rapid susceptibility determination from bacteria with minimal culturing (~105 per mL). This unique coupling of microfluidic cell separation with RNA-based molecular detection techniques represents significant progress towards faster diagnostics (~8 hours) to guide antibiotic therapy.

Graphical abstract: Direct detection and drug-resistance profiling of bacteremias using inertial microfluidics

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Publication details

The article was received on 16 Mar 2015, accepted on 09 Apr 2015 and first published on 17 Apr 2015


Article type: Paper
DOI: 10.1039/C5LC00311C
Citation: Lab Chip, 2015,15, 2297-2307

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    Direct detection and drug-resistance profiling of bacteremias using inertial microfluidics

    H. W. Hou, R. P. Bhattacharyya, D. T. Hung and J. Han, Lab Chip, 2015, 15, 2297
    DOI: 10.1039/C5LC00311C

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