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Issue 24, 2014
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Microfluidic platform generates oxygen landscapes for localized hypoxic activation

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Abstract

An open-well microfluidic platform generates an oxygen landscape using gas-perfused networks which diffuse across a membrane. The device enables real-time analysis of cellular and tissue responses to oxygen tension to define how cells adapt to heterogeneous oxygen conditions found in the physiological setting. We demonstrate that localized hypoxic activation of cells elicited specific metabolic and gene responses in human microvascular endothelial cells and bone marrow-derived mesenchymal stem cells. A robust demonstration of the compatibility of the device with standard laboratory techniques demonstrates the wide utility of the method. This platform is ideally suited to study real-time cell responses and cell–cell interactions within physiologically relevant oxygen landscapes.

Graphical abstract: Microfluidic platform generates oxygen landscapes for localized hypoxic activation

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Publication details

The article was received on 18 Jul 2014, accepted on 07 Oct 2014 and first published on 07 Oct 2014


Article type: Paper
DOI: 10.1039/C4LC01168F
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Citation: Lab Chip, 2014,14, 4688-4695

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    Microfluidic platform generates oxygen landscapes for localized hypoxic activation

    M. L. Rexius-Hall, G. Mauleon, A. B. Malik, J. Rehman and D. T. Eddington, Lab Chip, 2014, 14, 4688
    DOI: 10.1039/C4LC01168F

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