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Issue 24, 2014
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A simple microfluidic dispenser for single-microparticle and cell samples

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Abstract

Non-destructive isolation of single-cells has become an important need for many biology research laboratories; however, there is a lack of easily employed and inexpensive tools. Here, we present a single-particle sample delivery approach fabricated from simple, economical components that may address this need. In this, we employ unique flow and timing strategies to bridge the significant force and length scale differences inherent in transitioning from single particle isolation to delivery. Demonstrating this approach, we use an optical trap to isolate individual microparticles and red blood cells that are dispensed within separate 50 μl droplets off a microfluidic chip for collection into microscope slides or microtiter plates.

Graphical abstract: A simple microfluidic dispenser for single-microparticle and cell samples

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Supplementary files

Article information


Submitted
24 Jul 2014
Accepted
03 Oct 2014
First published
06 Oct 2014

Lab Chip, 2014,14, 4673-4679
Article type
Paper
Author version available

A simple microfluidic dispenser for single-microparticle and cell samples

A. Kasukurti, C. D. Eggleton, S. A. Desai, D. I. Disharoon and D. W. M. Marr, Lab Chip, 2014, 14, 4673
DOI: 10.1039/C4LC00863D

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