Jump to main content
Jump to site search

Issue 16, 2014
Previous Article Next Article

Bacterial chemotaxis on SlipChip

Author affiliations


This paper describes a simple and reusable microfluidic SlipChip device for studying bacterial chemotaxis based on free interface diffusion. The device consists of two glass plates with reconfigurable microwells and ducts, which can set up 20 parallel chemotaxis units as duplicates. In each unit, three nanoliter microwells and connecting ducts were assembled for pipette loading of a chemoeffector solution, bacterial suspension, and 1X PBS buffer solution. By a simple slipping operation, three microwells were disconnected from other units and interconnected by the ducts, which allowed the formation of diffusion concentration gradients of the chemoeffector for inducing cell migration from the cell microwell towards the other two microwells. The migration of cells in the microwells was monitored and accurately counted to evaluate chemotaxis. Moreover, the migrated cells were easily collected by pipetting for further studies after a slip step to reconnect the chemoeffector microwells. The performance of the device was characterized by comparing chemotaxis of two Escherichia coli species, using aspartic acid as the attractant and nitrate sulfate as the repellent. It also enables the separation of bacterial species from a mixture, based on the difference of chemotactic abilities, and collection of the cells with strong chemotactic phenomena for further studies off the chip.

Graphical abstract: Bacterial chemotaxis on SlipChip

Back to tab navigation

Supplementary files

Publication details

The article was received on 18 Feb 2014, accepted on 21 May 2014 and first published on 21 May 2014

Article type: Paper
DOI: 10.1039/C4LC00213J
Author version
Download author version (PDF)
Citation: Lab Chip, 2014,14, 3074-3080

  •   Request permissions

    Bacterial chemotaxis on SlipChip

    C. Shen, P. Xu, Z. Huang, D. Cai, S. Liu and W. Du, Lab Chip, 2014, 14, 3074
    DOI: 10.1039/C4LC00213J

Search articles by author