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Issue 13, 2014
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Phaseguide assisted liquid lamination for magnetic particle-based assays

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Abstract

We have developed a magnetic particle-based assay platform in which functionalised magnetic particles are transferred sequentially through laminated volumes of reagents and washing buffers. Lamination of aqueous liquids is achieved via the use of phaseguide technology; microstructures that control the advancing air–liquid interface of solutions as they enter a microfluidic chamber. This allows manual filling of the device, eliminating the need for external pumping systems, and preparation of the system requires only a few minutes. Here, we apply the platform to two on-chip strategies: (i) a one-step streptavidin–biotin binding assay, and (ii) a two-step C-reactive protein immunoassay. With these, we demonstrate how condensing multiple reaction and washing processes into a single step significantly reduces procedural times, with both assay procedures requiring less than 8 seconds.

Graphical abstract: Phaseguide assisted liquid lamination for magnetic particle-based assays

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Publication details

The article was received on 31 Jan 2014, accepted on 25 Apr 2014 and first published on 25 Apr 2014


Article type: Paper
DOI: 10.1039/C4LC00139G
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Citation: Lab Chip, 2014,14, 2334-2343
  • Open access: Creative Commons BY-NC license
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    Phaseguide assisted liquid lamination for magnetic particle-based assays

    C. Phurimsak, E. Yildirim, M. D. Tarn, S. J. Trietsch, T. Hankemeier, N. Pamme and P. Vulto, Lab Chip, 2014, 14, 2334
    DOI: 10.1039/C4LC00139G

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