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Issue 3, 2014
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Dielectrophoretically-assisted electroporation using light-activated virtual microelectrodes for multiple DNA transfection

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Abstract

Gene transfection is an important technology for various biological applications. The exogenous DNA is commonly delivered into cells by using a strong electrical field to form transient pores in cellular membranes. However, the high voltage required in this electroporation process may cause cell damage. In this study, a dielectrophoretically-assisted electroporation was developed by using light-activated virtual microelectrodes in a new microfluidic platform. The DNA electrotransfection used a low applied voltage and an alternating current to enable electroporation and transfection. Single or triple fluorescence-carrying plasmids were effectively transfected into various types of mammalian cells, and the fluorescent proteins were successfully expressed in live transfected cells. Moreover, the multi-triangle optical pattern that was projected onto a photoconductive layer to generate localized non-uniform virtual electric fields was found to have high transfection efficiency. The developed dielectrophoretically-assisted electroporation platform may provide a simpler system for gene transfection and could be widely applied in many biotechnological fields.

Graphical abstract: Dielectrophoretically-assisted electroporation using light-activated virtual microelectrodes for multiple DNA transfection

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Publication details

The article was received on 27 Sep 2013, accepted on 08 Nov 2013 and first published on 08 Nov 2013


Article type: Paper
DOI: 10.1039/C3LC51102B
Lab Chip, 2014,14, 592-601

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    Dielectrophoretically-assisted electroporation using light-activated virtual microelectrodes for multiple DNA transfection

    C. Wang, Y. Lee, H. Kuo, W. Liang, W. Li and G. Lee, Lab Chip, 2014, 14, 592
    DOI: 10.1039/C3LC51102B

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