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Issue 21, 2013
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Ultrasensitive microfluidic solid-phase ELISA using an actuatable microwell-patterned PDMS chip

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Abstract

Quantitative detection of low abundance proteins is of significant interest for biological and clinical applications. Here we report an integrated microfluidic solid-phase ELISA platform for rapid and ultrasensitive detection of proteins with a wide dynamic range. Compared to the existing microfluidic devices that perform affinity capture and enzyme-based optical detection in a constant channel volume, the key novelty of our design is two-fold. First, our system integrates a microwell-patterned assay chamber that can be pneumatically actuated to significantly reduce the volume of chemifluorescent reaction, markedly improving the sensitivity and speed of ELISA. Second, monolithic integration of on-chip pumps and the actuatable assay chamber allow programmable fluid delivery and effective mixing for rapid and sensitive immunoassays. Ultrasensitive microfluidic ELISA was demonstrated for insulin-like growth factor 1 receptor (IGF-1R) across at least five orders of magnitude with an extremely low detection limit of 21.8 aM. The microwell-based solid-phase ELISA strategy provides an expandable platform for developing the next-generation microfluidic immunoassay systems that integrate and automate digital and analog measurements to further improve the sensitivity, dynamic ranges, and reproducibility of proteomic analysis.

Graphical abstract: Ultrasensitive microfluidic solid-phase ELISA using an actuatable microwell-patterned PDMS chip

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Publication details

The article was received on 28 Jun 2013, accepted on 06 Aug 2013 and first published on 07 Aug 2013


Article type: Paper
DOI: 10.1039/C3LC50783A
Lab Chip, 2013,13, 4190-4197

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    Ultrasensitive microfluidic solid-phase ELISA using an actuatable microwell-patterned PDMS chip

    T. Wang, M. Zhang, D. D. Dreher and Y. Zeng, Lab Chip, 2013, 13, 4190
    DOI: 10.1039/C3LC50783A

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