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Issue 22, 2013
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Design of a single-step immunoassay principle based on the combination of an enzyme-labeled antibody release coating and a hydrogel copolymerized with a fluorescent enzyme substrate in a microfluidic capillary device

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Abstract

A combination of an enzyme-labeled antibody release coating and a novel fluorescent enzyme substrate-copolymerized hydrogel in a microchannel for a single-step, no-wash microfluidic immunoassay is demonstrated. This hydrogel discriminates the free enzyme-conjugated antibody from an antigen–enzyme-conjugated antibody immunocomplex based on the difference in molecular size. A selective and sensitive immunoassay, with 10–1000 ng mL−1 linear range, is reported.

Graphical abstract: Design of a single-step immunoassay principle based on the combination of an enzyme-labeled antibody release coating and a hydrogel copolymerized with a fluorescent enzyme substrate in a microfluidic capillary device

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Article information


Submitted
27 Jun 2013
Accepted
19 Aug 2013
First published
19 Aug 2013

Lab Chip, 2013,13, 4304-4307
Article type
Communication

Design of a single-step immunoassay principle based on the combination of an enzyme-labeled antibody release coating and a hydrogel copolymerized with a fluorescent enzyme substrate in a microfluidic capillary device

H. Wakayama, T. G. Henares, K. Jigawa, S. Funano, K. Sueyoshi, T. Endo and H. Hisamoto, Lab Chip, 2013, 13, 4304
DOI: 10.1039/C3LC50775K

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