Jump to main content
Jump to site search

Issue 22, 2013
Previous Article Next Article

Oligonucleotide-linked gold nanoparticle aggregates for enhanced sensitivity in lateral flow assays

Author affiliations

Abstract

Lateral flow assays (LFAs) as rapid analytical techniques promise to be widely used in point-of-care (POC) diagnostics because of their affordability and simplicity. However, LFAs still suffer from low sensitivity in detection of various biomarkers, e.g., nucleic acids. In this study, we developed a simple and general one-step signal amplification strategy, which employed oligonucleotide-linked gold nanoparticle (AuNP) aggregates to enhance the sensitivity in nucleic acid lateral flow (NALF) assays. Using a nucleic acid sequence of human immunodeficiency virus type 1 (HIV-1) as a model analyte, we observed that the detection limit of the developed NALF assay was 0.1 nM, which was improved by 2.5-fold compared with that of a non-signal amplification approach. The methodology described here could be used to detect a broad range of nucleic acids, and the general signal amplification approach could be potentially adopted in other types of LFAs.

Graphical abstract: Oligonucleotide-linked gold nanoparticle aggregates for enhanced sensitivity in lateral flow assays

Back to tab navigation

Supplementary files

Article information


Submitted
03 Jun 2013
Accepted
07 Aug 2013
First published
14 Aug 2013

Lab Chip, 2013,13, 4352-4357
Article type
Paper

Oligonucleotide-linked gold nanoparticle aggregates for enhanced sensitivity in lateral flow assays

J. Hu, L. Wang, F. Li, Y. L. Han, M. Lin, T. J. Lu and F. Xu, Lab Chip, 2013, 13, 4352
DOI: 10.1039/C3LC50672J

Social activity

Search articles by author

Spotlight

Advertisements