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Issue 18, 2013
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Microfluidic integrated optoelectronic tweezers for single-cell preparation and analysis

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Abstract

We report a novel microfluidic integrated optoelectronic tweezers (OET) platform for single-cell sample preparation and analysis. Integration of OET and microfluidics is achieved by embedding single-wall carbon nanotube (SWNT) electrodes into multilayer PDMS structures. This integrated platform allows users to selectively pick up individual cells from a population with light beams based on their optical signatures such as size, shape, and fluorescence, and transport them into isolated chambers using light induced dielectrophoretic forces. Isolated cells can be encapsulated into nanoliter liquid plugs and transported out of the platform for downstream molecule analysis using standard commercial instruments.

Graphical abstract: Microfluidic integrated optoelectronic tweezers for single-cell preparation and analysis

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Article information


Submitted
17 May 2013
Accepted
11 Jul 2013
First published
12 Jul 2013

Lab Chip, 2013,13, 3721-3727
Article type
Paper

Microfluidic integrated optoelectronic tweezers for single-cell preparation and analysis

K. Huang, Y. Wu, J. Lee and P. Chiou, Lab Chip, 2013, 13, 3721
DOI: 10.1039/C3LC50607J

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