Issue 18, 2013
From the journal:

Lab on a Chip

Ratchet nanofiltration of DNA

Joel D. P. Thomas,a   Mark N. Joswiak,ab   Daniel W. Olson,a   Sung-Gyu Parkac  and  Kevin D. Dorfman*a  

* Corresponding authors

a Department of Chemical Engineering and Materials Science, University of Minnesota – Twin Cities, 421 Washington Ave SE, Minneapolis, MN 55455, USA
E-mail: dorfman@umn.edu

b Department of Chemical Engineering, University of California – Santa Barbara, Santa Barbara, CA 93106, USA

c Advanced Functional Thin Films Department, Korea Institute of Materials Science, Changwon, Korea

Abstract

The DNA nanofilter is a microfabricated electrophoretic separation device consisting of a periodic array of thin slits (circa 60 nm) separated by deeper wells (circa 320 nm). We demonstrate that this device can act as a tuneable, clog-free filter when operating in a low frequency, asymmetric field inversion mode. This filtration occurs by using asymmetric field inversion to achieve bi-directional migration of short (less than 1000 bp) DNA. Moreover, similar ratchet-type operation can improve separations when compared to a constant field separation in the same device. These modes of operation enhance the utility of the DNA nanofilter as a component of integrated lab-on-a-chip devices. The experimental data confirm theoretical predictions for the bidirectional transport of DNA in entropy-based separations.

Graphical abstract: Ratchet nanofiltration of DNA

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Article information

DOI
https://doi.org/10.1039/C3LC50496D
Article type
Paper
Submitted
19 Apr 2013
Accepted
12 Jul 2013
First published
15 Jul 2013

Lab Chip, 2013,13, 3741-3746

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Ratchet nanofiltration of DNA

J. D. P. Thomas, M. N. Joswiak, D. W. Olson, S. Park and K. D. Dorfman, Lab Chip, 2013, 13, 3741 DOI: 10.1039/C3LC50496D

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