Jump to main content
Jump to site search

Issue 15, 2013

Microfluidic cytometer for high-throughput measurement of photosynthetic characteristics and lipid accumulation in individual algal cells

Author affiliations

Abstract

Commercially viable algal biofuel production requires discovery of new strains, genetic engineering for higher productivity and optimization of growth conditions. To accelerate research in these areas, we developed a microfluidic cytometer that measures forward light scatter, chlorophyll fluorescence induction and lipophilic stain fluorescence at a rate of 100 cells s−1. The chlorophyll fluorescence data is processed in real-time to measure the fluorescence-based maximum quantum yield, reported as Fv/Fm, to quantify the photochemical energy conversion of each cell. To demonstrate instrument performance, Fv/Fm measurements are obtained for unstressed (nutrient-replete) and stressed (nutrient-limited) cultures of the marine diatom Phaeodactylum tricornutum and are correlated to values obtained in bulk samples using traditional pulse-amplitude-modulating fluorometry. We then use the cytometer to characterize unstressed and stressed P. tricornutum and show that lipid content (as measured by Nile Red fluorescence) is inversely correlated with Fv/Fm. We believe these findings to be the first time that both photosynthetic efficiency and lipid accumulation have been simultaneously evaluated at the single cell level, and that in doing so, the diversity within these populations was revealed.

Graphical abstract: Microfluidic cytometer for high-throughput measurement of photosynthetic characteristics and lipid accumulation in individual algal cells

Supplementary files

Article information


Submitted
30 Dec 2012
Accepted
08 May 2013
First published
10 May 2013

Lab Chip, 2013,13, 2893-2901
Article type
Paper

Microfluidic cytometer for high-throughput measurement of photosynthetic characteristics and lipid accumulation in individual algal cells

R. A. Erickson and R. Jimenez, Lab Chip, 2013, 13, 2893 DOI: 10.1039/C3LC41429A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements