Jump to main content
Jump to site search

Issue 1, 2013
Previous Article Next Article

Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes

Author affiliations

Abstract

Here we show the fabrication of the Luminometric Sub-nanoliter Droplet-to-droplet Array (LUMDA chip) by inkjet printing. The chip is easy to be implemented and allows for a multiplexed multi-step biochemical assay in sub-nanoliter liquid spots. This concept is here applied to the integral membrane enzyme CYP3A4, i.e. the most relevant enzymatic target for phase I drug metabolism, and to some structurally-related inhibitors.

Graphical abstract: Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes

Back to tab navigation

Supplementary files

Article information


Submitted
19 Aug 2012
Accepted
25 Oct 2012
First published
26 Oct 2012

Lab Chip, 2013,13, 68-72
Article type
Technical Innovation

Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes

G. Arrabito, C. Galati, S. Castellano and B. Pignataro, Lab Chip, 2013, 13, 68
DOI: 10.1039/C2LC40948H

Social activity

Search articles by author

Spotlight

Advertisements