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Issue 21, 2012
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Open-cell recording of action potentials using active electrode arrays

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Abstract

The investigation of complex communication in cellular networks requires superior measurement tools than those available to date. Electrode arrays integrated onto silicon electronics are increasingly used to measure the electrical activity of cells in an automated and highly parallelized fashion, but they are restricted to recording extracellular potentials. Here, we report on an array of TiN electrodes built using standard silicon electronics for intracellular action potential recording. Intracellular access, possible at each of the 16 384 electrodes on the chip, was accomplished by local membrane electroporation using electrical stimulation with subcellular, micrometer-sized electrodes. Access to the cell interior was transient and could be tuned in duration by adapting the electroporation protocol. Intracellular sensing was found to be minimally invasive in the short and long-term, allowing consecutive intracellular recordings from the same cell over the course of days. Finally, we applied this method to investigate the effect of an ion channel blocker on cardiac electrical activity. This technique opens the door to massively parallel, long-term intracellular recording for fundamental electrophysiology and drug screening.

Graphical abstract: Open-cell recording of action potentials using active electrode arrays

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Publication details

The article was received on 17 Apr 2012, accepted on 02 Aug 2012 and first published on 06 Aug 2012


Article type: Paper
DOI: 10.1039/C2LC40656J
Citation: Lab Chip, 2012,12, 4397-4402

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    Open-cell recording of action potentials using active electrode arrays

    D. Braeken, D. Jans, R. Huys, A. Stassen, N. Collaert, L. Hoffman, W. Eberle, P. Peumans and G. Callewaert, Lab Chip, 2012, 12, 4397
    DOI: 10.1039/C2LC40656J

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