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Issue 19, 2012
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Nuclear deformation during breast cancer cell transmigration

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Abstract

Metastasis is the main cause of cancer mortality. During this process, cancer cells dislodge from a primary tumor, enter the circulation and form secondary tumors in distal organs. It is poorly understood how these cells manage to cross the tight syncytium of endothelial cells that lines the capillaries. Such capillary transmigration would require a drastic change in cell shape. We have therefore developed a microfluidic platform to study the transmigration of cancer cells. The device consists of an array of microchannels mimicking the confined spaces encountered. A thin glass coverslip bottom allows high resolution imaging of cell dynamics. We show that nuclear deformation is a critical and rate-limiting step for transmigration of highly metastatic human breast cancer cells. Transmigration was significantly reduced following the treatment with a protein methyltransferase inhibitor, suggesting that chromatin condensation might play an important role. Since transmigration is critical for cancer metastasis, this new platform may be useful for developing improved cancer therapies.

Graphical abstract: Nuclear deformation during breast cancer cell transmigration

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Submitted
01 May 2012
Accepted
17 Jul 2012
First published
18 Jul 2012

Lab Chip, 2012,12, 3774-3778
Article type
Paper

Nuclear deformation during breast cancer cell transmigration

Y. Fu, L. K. Chin, T. Bourouina, A. Q. Liu and A. M. J. VanDongen, Lab Chip, 2012, 12, 3774
DOI: 10.1039/C2LC40477J

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