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Issue 17, 2012
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Microfluidic platform for on-demand generation of spatially indexed combinatorial droplets

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Abstract

We propose a highly versatile and programmable nanolitre droplet-based platform that accepts an unlimited number of sample plugs from a multi-well plate, performs digitization of these sample plugs into smaller daughter droplets and subsequent synchronization-free, robust injection of multiple reagents into the sample daughter droplets on-demand. This platform combines excellent control of valve-based microfluidics with the high-throughput capability of droplet microfluidics. We demonstrate the functioning of a proof-of-concept device which generates combinatorial mixture droplets from a linear array of sample plugs and four different reagents, using food dyes to mimic samples and reagents. Generation of a one dimensional array of the combinatorial mixture droplets on the device leads to automatic spatial indexing of these droplets, precluding the need to include a barcode in each droplet to identify its contents. We expect this platform to further expand the range of applications of droplet microfluidics to include applications requiring a high degree of multiplexing as well as high throughput analysis of multiple samples.

Graphical abstract: Microfluidic platform for on-demand generation of spatially indexed combinatorial droplets

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Supplementary files

Article information


Submitted
23 Apr 2012
Accepted
30 May 2012
First published
30 May 2012

Lab Chip, 2012,12, 3055-3062
Article type
Paper

Microfluidic platform for on-demand generation of spatially indexed combinatorial droplets

H. Zec, T. D. Rane and T. Wang, Lab Chip, 2012, 12, 3055
DOI: 10.1039/C2LC40399D

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