Jump to main content
Jump to site search

Issue 22, 2012
Previous Article Next Article

A low-cost, label-free DNA detection method in lab-on-chip format based on electrohydrodynamic instabilities, with application to long-range PCR

Author affiliations

Abstract

In order to evolve from a “chip in the lab” to a “lab on a chip” paradigm, there is still a strong demand for low-cost, portable detection technologies, notably for analytes at low concentrations. Here we report a new label-free DNA detection method with direct electronic read, and apply it to long-range PCR. This method uses a nonlinear electrohydrodynamic phenomenon: when subjected to high electric fields (typically above 100 V cm−1), suspensions of large polyelectrolytes, such as long DNA molecules, create “giant” dynamic concentration fluctuations. These fluctuations are associated with large conductivity inhomogeneities, and we use here a contact-mode local conductivity detector to detect these fluctuations. In order to decouple the detection electronics from the high voltage excitation one, an original “doubly symmetric” floating mode battery-operated detection scheme was developed. A wavelet analysis was then applied, to unravel from the chaotic character of the electohydrodynamic instabilities a scalar signal robustly reflecting the amplification of DNA. As a first proof of concept, we measured the products of the off-chip amplification of 10 kbp DNA from lambda phage DNA, achieving a sensitivity better than 100 fg DNA in the original 50 μl sample. This corresponds to the amplification products of less than 100 initial copies of target DNA. The companion enabling technologies developed to implement this new concept, i.e. the doubly symmetric contact conductivity detection and wavelet analysis, may also find various other applications in lab-on-chips.

Graphical abstract: A low-cost, label-free DNA detection method in lab-on-chip format based on electrohydrodynamic instabilities, with application to long-range PCR

Back to tab navigation

Supplementary files

Publication details

The article was received on 18 Apr 2012, accepted on 13 Jul 2012 and first published on 16 Jul 2012


Article type: Paper
DOI: 10.1039/C2LC40372B
Lab Chip, 2012,12, 4738-4747

  •   Request permissions

    A low-cost, label-free DNA detection method in lab-on-chip format based on electrohydrodynamic instabilities, with application to long-range PCR

    M. L. Y. Diakité, J. Champ, S. Descroix, L. Malaquin, F. Amblard and J. Viovy, Lab Chip, 2012, 12, 4738
    DOI: 10.1039/C2LC40372B

Search articles by author

Spotlight

Advertisements