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Issue 23, 2012
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High-content single-cell analysis on-chip using a laser microarray scanner

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High-content cellomic analysis is a powerful tool for rapid screening of cellular responses to extracellular cues and examination of intracellular signal transduction pathways at the single-cell level. In conjunction with microfluidics technology that provides unique advantages in sample processing and precise control of fluid delivery, it holds great potential to transform lab-on-a-chip systems for high-throughput cellular analysis. However, high-content imaging instruments are expensive, sophisticated, and not readily accessible. Herein, we report on a laser scanning cytometry approach that exploits a bench-top microarray scanner as an end-point reader to perform rapid and automated fluorescence imaging of cells cultured on a chip. Using high-content imaging analysis algorithms, we demonstrated multiplexed measurements of morphometric and proteomic parameters from all single cells. Our approach shows the improvement of both sensitivity and dynamic range by two orders of magnitude as compared to conventional epifluorescence microscopy. We applied this technology to high-throughput analysis of mesenchymal stem cells on an extracellular matrix protein array and characterization of heterotypic cell populations. This work demonstrates the feasibility of a laser microarray scanner for high-content cellomic analysis and opens up new opportunities to conduct informative cellular analysis and cell-based screening in the lab-on-a-chip systems.

Graphical abstract: High-content single-cell analysis on-chip using a laser microarray scanner

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Supplementary files

Article information

01 Apr 2012
28 Aug 2012
First published
29 Aug 2012

Lab Chip, 2012,12, 5025-5033
Article type

High-content single-cell analysis on-chip using a laser microarray scanner

J. Zhou, Y. Wu, S. Lee and R. Fan, Lab Chip, 2012, 12, 5025
DOI: 10.1039/C2LC40309A

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