Issue 15, 2012

Functionalization of polycarbonate with proteins; open-tubular enzymatic microreactors

Abstract

This paper examines a set of techniques for the immobilization of enzymes on the surface of microchannels fabricated in polycarbonate (PC). Our experiments identify the method that uses combined physico-chemical immobilization on a layer of polyethyleneimine (PEI) as a reproducible vista for the robust immobilization of proteins. As an example, we demonstrate the fabrication, throughput and stability of an open-tubular reactor draped with alkaline phosphatase (ALP, EC 3.1.3.1) as a model enzyme. As PC is suitable for industrial applications the method could potentially be used to immobilize proteins in numbered-up implementations.

Graphical abstract: Functionalization of polycarbonate with proteins; open-tubular enzymatic microreactors

Supplementary files

Article information

Article type
Paper
Submitted
24 Feb 2012
Accepted
16 Apr 2012
First published
17 Apr 2012

Lab Chip, 2012,12, 2743-2748

Functionalization of polycarbonate with proteins; open-tubular enzymatic microreactors

D. Ogończyk, P. Jankowski and P. Garstecki, Lab Chip, 2012, 12, 2743 DOI: 10.1039/C2LC40204A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements