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Issue 7, 2012
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Rapid microarray processing using a disposable hybridization chamber with an integrated micropump

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Abstract

We present a disposable microarray hybridization chamber with an integrated micropump to speed up diffusion based reaction kinetics by generating convective flow. The time-to-result for the hybridization reaction was reduced from 60 min (standard protocol) down to 15 min for a commercially available microarray. The integrated displacement micropump is pneumatically actuated. It includes two active microvalves and is designed for low-cost, high volume manufacturing. The setup is made out of two microstructured polymer parts realized in polycarbonate (PC) separated by a 25 μm thermoplastic elastomer (TPE) membrane. Pump rate can be controlled between 0.3 μl s−1 and 5.7 μl s−1 at actuation frequencies between 0.2 Hz and 8.0 Hz, respectively.

Graphical abstract: Rapid microarray processing using a disposable hybridization chamber with an integrated micropump

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Publication details

The article was received on 15 Nov 2011, accepted on 25 Jan 2012 and first published on 27 Jan 2012


Article type: Paper
DOI: 10.1039/C2LC21110F
Citation: Lab Chip, 2012,12, 1384-1388

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    Rapid microarray processing using a disposable hybridization chamber with an integrated micropump

    J. Rupp, M. Schmidt, S. Münch, M. Cavalar, U. Steller, J. Steigert, M. Stumber, C. Dorrer, P. Rothacher, R. Zengerle and M. Daub, Lab Chip, 2012, 12, 1384
    DOI: 10.1039/C2LC21110F

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