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Issue 7, 2012
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A multifunctional pipette

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Microfluidics has emerged as a powerful laboratory toolbox for biologists, allowing manipulation and analysis of processes at a cellular and sub-cellular level, through utilization of microfabricated features at size-scales relevant to that of a single cell. In the majority of microfluidic devices, sample processing and analysis occur within closed microchannels, imposing restrictions on sample preparation and use. We present an optimized non-contact open-volume microfluidic tool to overcome these and other restrictions, through the use of a hydrodynamically confined microflow pipette, serving as a multifunctional solution handling and dispensing tool. The geometries of the tool have been optimised for use in optical microscopy, with integrated solution reservoirs to reduce reagent use, contamination risks and cleaning requirements. Device performance was characterised using both epifluorescence and total internal reflection fluorescence (TIRF) microscopy, resulting in ∼200 ms and ∼130 ms exchange times at ∼100 nm and ∼30 μm distances to the surface respectively.

Graphical abstract: A multifunctional pipette

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The article was received on 19 Sep 2011, accepted on 08 Dec 2011 and first published on 17 Jan 2012

Article type: Paper
DOI: 10.1039/C2LC20906C
Lab Chip, 2012,12, 1255-1261
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    A multifunctional pipette

    A. Ainla, G. D. M. Jeffries, R. Brune, O. Orwar and A. Jesorka, Lab Chip, 2012, 12, 1255
    DOI: 10.1039/C2LC20906C

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