Jump to main content
Jump to site search

Issue 12, 2012
Previous Article Next Article

A microfluidic device for whole-animal drug screening using electrophysiological measures in the nematode C. elegans

Author affiliations

Abstract

This paper describes the fabrication and use of a microfluidic device for performing whole-animal chemical screens using non-invasive electrophysiological readouts of neuromuscular function in the nematode worm, C. elegans. The device consists of an array of microchannels to which electrodes are attached to form recording modules capable of detecting the electrical activity of the pharynx, a heart-like neuromuscular organ involved in feeding. The array is coupled to a tree-like arrangement of distribution channels that automatically delivers one nematode to each recording module. The same channels are then used to perfuse the recording modules with test solutions while recording the electropharyngeogram (EPG) from each worm with sufficient sensitivity to detect each pharyngeal contraction. The device accurately reported the acute effects of known anthelmintics (anti-nematode drugs) and also correctly distinguished a specific drug-resistant mutant strain of C. elegans from wild type. The approach described here is readily adaptable to parasitic species for the identification of novel anthelmintics. It is also applicable in toxicology and drug discovery programs for human metabolic and degenerative diseases for which C. elegans is used as a model.

Graphical abstract: A microfluidic device for whole-animal drug screening using electrophysiological measures in the nematode C. elegans

  • This article is part of the themed collection: Focus on USA
Back to tab navigation

Supplementary files

Article information


Submitted
01 Jan 2012
Accepted
24 Apr 2012
First published
26 Apr 2012

Lab Chip, 2012,12, 2211-2220
Article type
Paper

A microfluidic device for whole-animal drug screening using electrophysiological measures in the nematode C. elegans

S. R. Lockery, S. E. Hulme, W. M. Roberts, K. J. Robinson, A. Laromaine, T. H. Lindsay, G. M. Whitesides and J. C. Weeks, Lab Chip, 2012, 12, 2211
DOI: 10.1039/C2LC00001F

Social activity

Search articles by author

Spotlight

Advertisements